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Inhibition of Tumor Necrosis Factor-{alpha} Improves Physiological Angiogenesis and Reduces Pathological Neovascularization in I


American Society for Investigative Pathology

Type: Report
Region: Northern Ireland

Tom A. Gardiner*, David S. Gibson*, Tanyth E. de Gooyer*, Vidal F. de la Cruz, Denise M. McDonald* and Alan W. Stitt From Ophthalmology and Vision Science,* Queen’s University Belfast, Belfast, Northern Ireland; and Cytokine PharmaSciences, Inc., King of Prussia, Pennsylvania The present study was undertaken to test whether inhibition of the proangiogenic inflammatory cytokine tumor necrosis factor(TNF)-{alpha} can modulate retinal hypoxia and preretinal neovascularization in a murine model of oxygen-induced retinopathy (OIR). OIR was produced in TNF-{alpha}–/– and wild-type (WT) control C57B6 neonatal mice by exposure to 75% oxygen between postnatal days 7 and 12 (P7 to P12). Half of each WT litter was treated with the cytokine inhibitor semapimod (formerly known as CNI-1493) (5 mg/kg) by daily intraperitoneal injection from the time of reintroduction to room air at P12 until P17. The extent of preretinal neovascularization and intraretinal revascularization was quantified by image analysis of retinal flat-mounts and retinal hypoxia correlated with vascularization by immunofluorescent localization of the hypoxia-sensitive drug pimonidazole (hypoxyprobe, HP). HP adducts were also characterized by Western analysis and quantified by competitive enzyme-linked immunosorbent assay. TNF- {alpha}–/–and WT mice showed a similar sensitivity to hyperoxia-induced retinal ischemia at P12. At P13 some delay in early reperfusionwas evident in TNF-{alpha} –/– and WT mice treated with semapimod. However, at P17 both these groups had significantly better vascular recovery with less ischemic/hypoxic retina and preretinal neovascularization compared to untreated retinopathy in WT mice. Immunohistochemistry showed deposition of HP in the avascular inner retina but not in areas underlying preretinal neovascularization,indicating that such aberrant vasculature can reduce retinal hypoxia. Inhibition of TNF-{alpha} significantlyimproves vascular recovery within ischemic tissue and reduces pathological neovascularization in OIR. HP provides a usefultool for mapping and quantifying tissue hypoxia in experimentalischemic retinopathy.



Rights: Public
Suggested citation:

American Society for Investigative Pathology. (2008) Inhibition of Tumor Necrosis Factor-{alpha} Improves Physiological Angiogenesis and Reduces Pathological Neovascularization in I [Online]. Available from: [Accessed: 17th June 2019].


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